Temporary

Part:BBa_K1484316:Design

Designed by: Salil Bhate   Group: iGEM14_Cambridge-JIC   (2014-10-08)


Mp Selection Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
    Illegal BglII site found at 235
    Illegal XhoI site found at 1480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 859
    Illegal NgoMIV site found at 923
    Illegal NgoMIV site found at 1065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1351


Design Notes

MoClo Sites


Source

Synth w/o sites DNA2.0

References